Gianluca Mancini, Matteo Pessina and Matteo Politano were cut from the squad.
“It’s always difficult to get to the list of 26, because you have to leave at home players who have nevertheless been protagonists,” said Mancini, who transformed Italy since taking over after the Azzurri failed to qualify for the 2018 World Cup.
“Our team has been reborn after the disappointment of the last World Cup and we have done well in building a good path.”
Italy is in Group A with Turkey, Switzerland and Wales for the European Championship. The opening game of the tournament features Italy against Turkey in Rome on June 11.
Italy hosts the Czech Republic on Friday in a friendly.
Defenders: Francesco Acerbi (Lazio), Alessandro Bastoni (Inter Milan), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Giovanni Di Lorenzo (Napoli), Emerson (Chelsea), Alessandro Florenzi (Paris Saint-Germain), Leonardo Spinazzola (Roma), Rafael Toloi (Atalanta)
Midfielders: Nicolò Barella (Inter Milan), Bryan Cristante (Roma), Jorginho (Chelsea), Manuel Locatelli (Sassuolo), Lorenzo Pellegrini (Roma), Stefano Sensi (Inter Milan), Marco Verratti (Paris Saint-Germain)
Forwards: Andrea Belotti (Torino), Domenico Berardi (Sassuolo), Federico Bernardeschi (Juventus), Federico Chiesa (Juventus), Ciro Immobile (Lazio), Lorenzo Insigne (Napoli), Giacomo Raspadori (Sassuolo)
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